Isolation and Antibiotics Resistance Pattern of Pseudomonas aeruginosa From Clinical and Environmental Samples in Owerri, Imo State

Pseudomonas aeruginosa is bacterium specie of Pseudomonas, one of the causes of nosocomial infection and resistance to many antibiotics. Pseudomonas aeruginosa has ability to survive in moist environments which makes it a common pathogen. It can cause Ocular infection, Ear infection, Wound infection, Urinary tract infection and few other rarely cause diseases. There is misinterpretation of Pseudomonas aeruginosa infection with Buruli ulcer locally called Acha-ere in Owerri Imo State and many Pseudomonas aeruginosa infections have been reported in severally in a particular hospital in Owerri Imo State. Pseudomonas aeruginosa was isolated using Nutrient agar and Centrimide Selective Media. Biochemical tests like Gram staining, Motility test, Oxidase test, Catalase test, Indole test, Coagulase test, Methyl red test, Voges Prostauer test and Triple Sugar Indole test enabled the identification of Pseudomonas aeruginosa from Clinical and Environmental samples. Environmental and Clinical samples used in this study include Water, Soil, Urine, Sputum, HVS, Blood, Hospital bathroom samples, Ward floor samples, Wound swab sample and Washing basin samples. 450 clinical samples collected, 30 samples were positive to Pseudomonas aeruginosa, 450 Hospital environmental samples collected, 56 were positive to Pseudomonas aeruginosa and 61 Environmental samples collected, 4 were positive to Pseudomonas aeruginosa. The results of this study shows the presence of Pseudomonas aeruginosa infection and they are resistance to most of the Cephalosporin antibiotics and Quinoline antibiotics.